Multi-scale computational study of the mechanical regulation of cell mitotic rounding in epithelia
Fig 7
Response surface method analysis of mechanical properties on regulating mitotic expansion and mitotic rounding.
(A) Schematic of initial full factorial design (FFD) for exploring parameter space, and subsequent central composite design (CCD) for developing the response surface models shown in (C, D). (B) Pareto front indicating computational model parameter values with lowest difference with experimental data for area ratio and normalized roundness. The parameter range defined by the CCD (Run 2) spans parameter variation where the error between experiments and simulations is within the propagated uncertainty of measurements and simulations. Error bars are the standard error of means of the normalized deviation between experiments and simulations. (C-D) Contour plots for FFD experiment where (C) shows the area ratio (Aratio = Amit/Ainter) and (D) shows the normalized roundness (Rnorm). (E-F) Contour plots for CCD experiment where (E) shows the area ratio (Aratio) and (F) shows the normalized roundness (Rnorm).