Multi-scale computational study of the mechanical regulation of cell mitotic rounding in epithelia
Fig 5
(A-A‴) Time-lapse confocal images of cell undergoing mitosis in the wing disc with E-cadherin:GFP-labeled cell boundaries. Scale bar is 5 μm. Arrows indicate daughter cells. (B-D‴) Time series from Epi-Scale simulation of a cell undergoing mitosis and division with illustration of: (B-B‴) adhesive spring stiffness, (C-C‴) cortical spring stiffness, and (D-D‴) internal pressure, respected to their interphase values. (E-F) Comparison of size and roundness of mitotic cells with experimental data for the Drosophila wing disc. Arrow represents mitotic cell in B-D. A t-test comparing the means of computational simulations and experiments result in p = 0.72 for cell area ratio and p = 0.76 for normalized roundness of mitotic cells.