Multi-scale computational study of the mechanical regulation of cell mitotic rounding in epithelia
Fig 4
Calibration of model parameters through simulations.
(A-A″) Calibration test to determine parameters for cell elasticity, analogous to experimental single cell stretching tests [66], (A) Initial condition t = 0, (A′) 6 minutes after simulation with no force applied, (A″) after 72 minutes cell is completely on tension (B-B″) Cell adhesivity test, analogous to experimental tests [69] for calibrating the level of cell-cell adhesion between adjacent cells. (B) Initial condition t = 0, (B′) 6 minutes after simulation begins with no force applied, (B″) after 72 minutes, 15 nN force is applied. (C) Stress versus strain for single cell calibration (red line) and stress versus strain for calibrating the level of adhesivity between the two cells (blue line) [69,70]. Initial negative strain in adhesivity test is due to strong adhesion between two cells. (D) Force and strain as a function of time for adhesivity test. (E) Tissue growth rate calibration by comparing with the experimental data by Wartlick et al. [56]. The 95% confidence interval for the growth rate results is shown in grey color.