An integrated calcium imaging processing toolbox for the analysis of neuronal population dynamics
Fig 5
User interface screenshots of the calculated neuronal responses associated with experimental events.
(A-E) Responses to visual stimulations with light spots at different azimuth angles of the larva’s visual field (two-photon imaging of a GCaMP3-expressing zebrafish larva). (A) ROIs are colored with an HSV color code representing their preferred azimuth angle (Peak mapping parameter; hue), azimuth selectivity (Tuning width; saturation) and average response at preferred azimuth (Response strength; value). Due to the skewed distribution of the responses, only a few responsive ROIs can be visualized. (B) Offsetting and clipping of the saturation and value channels to improve visualization. Black, original values used in A; red, rescaled values used in C. (C) Same data shown in A, but with the rescaled channel ranges. After this step, the retinotopic organization of the optic tectum becomes evident. (D and E) Screenshots of the responses of two ROIs selected by clicking on Select ROI in c. Average (black) and single-trial (gray) ΔF/F0 responses are organized according to the stimulus values, where significant trial responses are shown in red. Bottom right, tuning curves of the ROIs. Black, mean response; gray patch, standard error. Note how the responsive but less selective ROI in e is shown with a more whitish color code (low saturation). (F) Responses to visual stimulations with gratings moving in angular directions, monitored by volumetric light-sheet single-photon imaging of a GCaMP5-expressing zebrafish larva. Responses are displayed in HSV color code over the maximal intensity projection of all imaged optical sections. Note that, while the tectal neuropil indiscriminately responds to all directions (whitish ROIs), a pair of bilaterally symmetric group of ROIs in the hindbrain responds selectively to either 60° or -60° (see S1 Video for volumetric distribution of responses).