Probabilistic fluorescence-based synapse detection
Fig 14
Top Left: Histogram of a slice of the probability map. Top Middle: Thresholded probability map, see text for details on the threshold selection. Top right: Probability map of one slice of a 13 μ m ×17 μ m region of the AT data on [19]. Bottom Row:. A series of pseudo-color images indicating the presence of the receptors PSD-95 (red), VGluT1 (blue), and synapsin (green) across three consecutive slices. Centroids of detected synapses containing all three receptors (query) are circled in black.