Probabilistic fluorescence-based synapse detection
Fig 13
Synaptogram showing a ‘false negative.’ While the corresponding EM sections shows a synapse, there is insuficient synaptic IF signal available to justify the presence of a synapse using solely IF data. Again, the algorithm makes the same mistake a human expert would make when working only with the IF data. Each ‘block’ is 1.086μ m ×1.130μ m. As before, the bottom panel shows enlarged, consecutive slices of the EM data, which was used to manually annotate the synapse. The scale bar on the lower left side is 500 nm.