Probabilistic fluorescence-based synapse detection
Fig 7
Top: Three consecutive slices of 2D puncta probability, given by Eq (3). Bottom: Factor image given by Eq (4) (left) and the corresponding 3D puncta probability, given by Eq (5), (right) of the center slice in the top row. Only those 2D puncta that actually span multiple slices are kept with high intensity (probability) in the combined result (bottom right). The green arrow points to an example of a probable punctum that spans multiple slices. The red arrow points to an example of a relatively-less probable punctum which does not span multiple slices and therefore is diminished in the output image. Each image is a cutout of size 2261 × 2501 pixels or 5.268 × 5.827μ m.