Probabilistic fluorescence-based synapse detection
Fig 2
Three 1.277 μ m ×1.186 μ m images showing conjugate EM and IF data. The left panel has synapsin (green) and PSD-95 (red) data overlaid, marked by the colored boundary lines. The scale bar represents 500 nm. The presence of both presynaptic and postsynaptic channels indicates the presence of a synapse with high probability. The center panel shows the PSD-95 IF image and the right panel shows the synapsin IF image. On both images, the EM-identified synaptic cleft is marked by a blue box. While the proposed synapse detection method uses multiple synaptic markers, only two are shown here for visualization purposes.