Highly accessible AU-rich regions in 3’ untranslated regions are hotspots for binding of regulatory factors
Fig 6
RBPs compete with each other and with miRNAs for binding on hotspots.
Cumulative fraction plot showing the effect of having 0, 1 or 2 or more a) hisites b) HuR binding sites overlapping RBP hotspots. As an additional control genes lacking a) hisites and b) HuR binding sites are shown. The x-axis shows the distribution of log2FC upon a) AGO2 or b) HuR KD. The y-axis shows the cumulative fraction of transcripts. *, ** and *** denote a p-value < 0.05, 0.01 and 0.001 respectively. The sets containing 1 or more sites overlapping hotspots are compared to set without sites overlapping hotspots. The set without sites overlapping hotspots is compared to the control set.