Highly accessible AU-rich regions in 3’ untranslated regions are hotspots for binding of regulatory factors
Fig 3
RBP binding sites are enriched on target sites of highly expressed miRNAs.
a) Characteristic CLIP enrichment profiles for AGO2, HuR, EWSR1 and WDR33 RBPs around miRNA target sites that are highly (red), moderately (green) and lowly (blue) expressed. The grey and the black lines show the maximum and the minimum enrichment values for the 90% confidence intervals around random miRNA target sites. b) Heatmap showing the distribution of CLIP enrichment values around hisites. The colors range from red (high enrichment) to blue (low enrichment). c) Scatter plot summarizing the correlation values between RBP enrichment on miRNA target sites and miRNA expression (y-axis) and their p-values (x-axis). d) Barplot summarizing the fraction of RBP clusters on hisites (bars) and the fraction of hisites overlapped by RBPs (points). Colored bars (blue) and points (yellow) highlight the cases in which the fraction of RBPs or miRNA target sites is higher than expected by chance (empirical p-value < 0.01 using 100 random miRNA target site sets).