Modelling T cell proliferation: Dynamics heterogeneity depending on cell differentiation, age, and genetic background
Fig 8
Quantification of cell proliferation and death in thymus.
For each stage of thymocyte differentiation, the total number of live cells (green), of accumulated EdU+ (red) cells, and of dead cells (blue) after the pulse/chase/pulse are shown (upper panel). The percentage of EdU+ cells (red) and dead cells (blue) among each differentiation stage identified is given (lower panel). Quantification is done by FACS and given as the median (line) of 4 mice per group (points). In DN2 and DN3 cells in some B6 mice death is so rare that quantification is below 10 cells/thymus and sometimes up to 0 and cannot be represented on the log scale. The ratio of EdU+/dead cells gives a performance of cell expansion.