Modelling T cell proliferation: Dynamics heterogeneity depending on cell differentiation, age, and genetic background
Fig 3
State transition model of cell cycle and EdU labeling.
According to the FACS dot plot, the evolution of cells through the cell cycle and EdU labelling can be represented by a transition diagram. Unlabelled cells can transit successively in the three phases of the cell cycle: G0/G1 (G), S (S), and G2/M (M). Terms in aX correspond to rates of entry into the next phase of cells in X phase (X being either G, S, or M). The exit of cells, either due to death, differentiation, or migration is represented by terms in dX. During the pulse-phase the cells in S phase incorporate EdU (red arrow) and enter into S’ with rate β and continue the cycle to M’ and G’. During the chase phase, in the absence of EdU, the labelled cells lose labelling upon several cell divisions (blue arrow) (de-labelling) with rate α. Unlabelled cells can enter into S phase but remain unlabelled.