Metacoder: An R package for visualization and manipulation of community taxonomic diversity data
Fig 4
Flexible parsing and digital PCR allows for comparisons of primers and databases.
Shown is a comparison of digital PCR results for three 16S reference databases. The plots on the left display abundance of all bacterial 16S sequences. Plots on the right display all taxa with subtaxa not entirely amplified by digital PCR using universal 16S primers [18]. Node color and size display the proportion and number of sequences not amplified respectively.