Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
Fig 2
(A) Genome size and filtered protospacer density for the five species tested. (B) The fraction of protospacers passing filters of off-targeting, efficiency score, and both. The latter are defined as “filtered protospacers”, whose density is shown in (A). Data are displayed as a fraction of the total number of canonical PAM sequences in each genome. (C) The effect on library quality of modifying design variables. Y-axis denotes the percent of target regions, divided by: “successful”, where n = 10 distinct sgRNA pair designs are returned per target; “intermediate” designs, where 0<n<10 pairs are returned; “failed” designs, where n = 0 pairs are returned. CRISPETa was run on a test set of 7000 targets (see Materials and Methods for details). The first column represents the run performed with default settings, and in each subsequent column one variable is modified (see Table 3 for details).