Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
Fig 1
Overview of CRISPETa pipeline.
(A) Schematic of CRISPR-mediated genomic deletion. The aim is elimination of the Target region through recruitment of a pair of Cas9 proteins. Red boxes represent protospacers, the 20 bp upstream of a PAM and recognised by the sgRNA. (B) The CRISPETa workflow.