FIJI Macro 3D ART VeSElecT: 3D Automated Reconstruction Tool for Vesicle Structures of Electron Tomograms
Fig 4
Comparison of N2 and unc-59(e261) C. elegans tomograms using 3D ART VeSElecT.
Fig 4 shows steps of vesicle annotation using 3D ART VeSElecT on N2 (A-A”) and unc-59(e261) (B-B”) C. elegans tomograms. In (A, B) the initial tomogram is illustrated, (A’, B’) show the composite stack that includes the original tomogram plus the annotation of the vesicle pool (vesicles are shown in arbitrary colors). The three dimensional model of the semi-automated annotation (automated registration plus manual proof-reading) of the vesicle pool is shown in (A”, B”). In (C) the semi-automated vesicle pool annotation of (A’) was transferred to IMOD where further structures of the NMJ were complemented. Vesicles are shown in yellow, cell membranes are green, dense projections are blue, endoplasmatic reticulum is white and microtubules are in shades of purple. In (D) synaptic vesicle sizes of N2 and unc-59(e261) were compared. Figure (E) illustrates the comparison of vesicle pool density by determining the three closest vesicles (N2 is illustrated in dark blue, unc-59(e261) is illustrated in light blue). In (F) the approach for determination of the vesicle pool density is illustrated. It is shown that for each vesicle the distance of its center to the center of its three closest neighbors is calculated. (scale bars = 100 nm).