Real Time Multiplicative Memory Amplification Mediated by Whole-Cell Scaling of Synaptic Response in Key Neurons
Fig 1
CaMKII blockers (KN93 left; tatCN21 right) act through a whole-cell, two-fold division of the GABAA single channel conductance in a subset of cells from trained rats.
A, E: Depicted for each cell is the effect of CaMKII blocker on the average and standard deviation of the event amplitudes. The effect of the CaMKII blocker clearly exhibits two clusters, where one cluster exhibits a large blocker effect on the average and standard deviation of the event amplitude (KN93: trained, n = 16; pseudo, n = 8; naïve, n = 10. tatCN21: trained, n = 10; pseudo, n = 7; naive, n = 5) B, F: Similarly, the effect of CaMKII blocker on the average event amplitude and single channel current exhibits two clusters (KN93: trained, n = 12; pseudo, n = 6; naive, n = 5. tatCN21: trained, n = 10; pseudo, n = 4; naïve, n = 5). C, G: An amplitude distribution curve averaged for all cells in the affected cluster (all cells in the blocker affected cluster for which the single channel current could be calculated; KN93: n = 5; tatCN21: n = 4) is shown for the event amplitudes recorded before (purple) and after (orange) CaMKII blocker. The blocker effect was computationally reversed (black curve) by applying the reverse calculation on the data recorded after applying the CaMKII blocker: The fraction of events that were affected by the drug was calculated (Materials and Methods). This fraction of events was randomly selected from the events recorded after the blocker was applied and the amplitude of these events was multiplied by a factor of two. A new amplitude distribution curve was calculated from the whole set of data, using both the multiplied and the non-multiplied events, exhibiting a fully computationally reversed CaMKII blocker effect. The P values were calculated using Cramer Von Mises two-sample test. D, H: the blocker effect could be reversed in each cell separately. Left: an example of one cell where the histogram of events amplitudes before CaMKII blocker (blue) matched the histogram of the calculated events amplitude after the blocker effect was computationally reversed (red), right the P value was calculated for each cell and the average of these P values was calculated from all cells in the highly affected cluster.