An Atlas of Peroxiredoxins Created Using an Active Site Profile-Based Approach to Functionally Relevant Clustering of Proteins
Fig 4
MISST and PSSM Analysis flowcharts describe the process of agglomerative identification of sequences as members of functionally relevant groups.
(A) Flow chart of the MISST process for identifying functionally relevant groups within a protein superfamily. (B) An illustration of the agreement criterion: a scatterplot of all proteins identified by DASP2 searches using two ASPs, Group 4A and Group 4B, that were subdivided in the previous MISST iteration. Red lines indicate the significance threshold used to label proteins as “significant” or “not significant” in each group. Sequences in the yellow quadrants are those identified in both searches at similar (significant or not) DASP2 scores. Those sequences in the cyan quadrants differ in significance. This metric is used to determine if a group that is subdivided by PSSM Analysis produces truly distinct search results. (C) Flow chart of PSSM Analysis for identifying when and how to divide clusters into functionally relevant groups.