Precision of Readout at the hunchback Gene: Analyzing Short Transcription Time Traces in Living Fly Embryos
Fig 6
Longer time traces help distinguish between two state and three state cycle models.
A. Inference from data generated by a two state model, which corresponds to k1/k2 = 0, from traces of different lengths T and using different numbers of nuclei N shows that longer traces help increase the probability to correctly learn the model type. Increasing the number of nuclei for short traces shows little improvement. The inference is repeated 50 times per condition. The experimental conditions studied in this paper are closest to the T = 240s and N = 50 nuclei panel. B. The same numerical experiment but assuming a three state cycle model, which corresponds to k1/k2 = 1. Parameters of the simulations: Pon = 0.1, koff + 1/(1/k1 + 1/k2) = 0.02s−1 and k1/k2 = 0 in A, and k1/k2 = 1 in B.