The Protein Cost of Metabolic Fluxes: Prediction from Enzymatic Rate Laws and Cost Minimization
Fig 8
Data integration in the ECM-based modeling workflow.
After collecting all available kinetic and thermodynamic data and mapping them onto the network model, we use parameter balancing to obtain a consistent, complete set of kinetic constants. For a fully parameterized kinetic model, the metabolite and enzyme levels must be determined. We compute them by enzyme cost minimization with predefined metabolic fluxes (obtained from experiments or computationally). Finally, the predicted values are validated with measured metabolite and protein concentrations.