Qualitative Dynamical Modelling Can Formally Explain Mesoderm Specification and Predict Novel Developmental Phenotypes
Fig 4
Simulations of known genetic perturbations.
The results of selected simulations of loss-of-function (lof), gain-of-function (gof) mutations, and of combination thereof are shown in the form of coloured square vignettes, along with references to articles presenting matching data. The first vignette (top left) correspond to the wild type situation, with VM, H, FB and SM presumptive territories coloured in blue, red, green and orange, respectively. In the following vignettes, the coloration of the four presumptive territories are modified to reflect the absence or important markers, or the combination of markers associated with different tissues. Wg lof leads to the loss of Wg/Slp domain, resulting in an expansion of the En/Hh domain; consequently, the model correctly predicts the loss of H along with a potential perturbation of SM (yellow domains). Dpp lof leads to the loss of dorsal derivatives (VM and H), along with an expansion of FB. Dpp gof leads to an expansion of VM at the expense of FB, along with a perturbation of SM. Tin lof shows a loss of dorsal tissues, while Bap lof exhibits only the loss of VM. Finally, the combination of Wg gof and Hh lof leads to a dorsal expansion of H, along with a loss of FB, while the combination of Dpp gof, Hh gof and Wg lof leads to an expansion of VM in the whole mesoderm.