Identification of Cell Type-Specific Differences in Erythropoietin Receptor Signaling in Primary Erythroid and Lung Cancer Cells
Fig 2
Example model with simulated data for two different cell types.
The process diagram of a two-step phosphorylation reaction (Protein→pProtein→ppProtein) is shown according to Systems Biology Graphical Notation. The ODE was numerically solved over time for two cell types that differ in the initial protein concentration ([Protein]t = 0) and in one kinetic rate (k3). The initial concentrations of the phosphorylated compounds were set to zero ([pProtein]t = 0 = [ppProtein]t = 0 = 0). The second phosphorylation step is reversible and the dephosphorylation rate (k3) was assumed to be cell type-specific. The parameters for the phosphorylation steps (k1, k2) are the same for both cell types. Simulated data points are depicted as black dots, and the grey shading indicates the standard deviation (σ = 0.1) of the simulated measurement errors. Model trajectories are displayed as black lines.