Integrating Transcriptomic and Proteomic Data Using Predictive Regulatory Network Models of Host Response to Pathogens
Fig 1
Integrative inference of regulatory networks controlling host response to influenza infection.
The main components of our approach are: (1) MERLIN to learn regulatory networks and modules from genome-wide host mRNA profiles from multiple independent virus infections, (2) Multi-Task Group LASSO (MTG-LASSO) approach to predict protein-level regulators (dark blue squares) of mRNA-based modules, (3) construction of active network components for different virus strains and time points, (4) siRNA-based validation of predicted regulators, (5) predicting upstream signaling networks for each module by identifying minimal physical subnetworks that connect module regulators (dark blue squares and light blue ellipses) with a small number of intermediate nodes (yellow diamonds).