Cellular Interrogation: Exploiting Cell-to-Cell Variability to Discriminate Regulatory Mechanisms in Oscillatory Signalling
Fig 2
Cellular responses to steps and pulses.
(A) Plots of two cells (identified in the top right-hand corner by experiment number and cell number as listed in S1 Text), showing effective Ca2+ concentration in the cytoplasm as a function of time, in response to a step of 10 μM histamine, illustrated in the graph on the left and also beneath each plot in red. The inset shows a histogram of mean inter-spike periods over all cells for three step experiments (experiment numbers 1–3 in S1 Text), with the green bar showing a mean ± SD of 130 ± 40. (B) Plots of two cells, as previously, in response to repetitive pulses of 10 μM histamine with a pulse width of 22 seconds and an inter-pulse period of 60 seconds, as defined in the graph on the left.