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EGFR Signal-Network Reconstruction Demonstrates Metabolic Crosstalk in EMT

Fig 5

EGFR overexpression in D492M.

(A) Real-Time Quantitative Reverse Transcription PCR of EGFR in D492, D492M^EGFR and D492M^Empty, normalized to GAPDH. EGFR transcription level is significantly higher in D492M^EGFR compared to D492M^Empty but does not reach the D492 EGFR transcription level. (B) Protein expression of EGFR, Phospho-p44/42 MAPK (ERK1/2) and Phospho-AKT determined by Western blotting. Overexpression of EGFR in D492M leads to increased protein expression of EGFR in D492M^EGFR compared to D492M^Empty, but EGFR protein expression does not reach the D492 level. Overexpression of EGFR in D492M leads to increased MAPK (Erk1/2) and Akt phosphorylation which is higher than in both D492M^Empty and D492.

doi: https://doi.org/10.1371/journal.pcbi.1004924.g005