EGFR Signal-Network Reconstruction Demonstrates Metabolic Crosstalk in EMT
Fig 3
The pipeline employed to generate context specific signaling networks EGFR_E and EGFR_M of an immortalized breast epithelial cell line (D492) and mesenchymal cell line (D492M).
SBML pathway map from Reactome was converted to mathematical format using the COBRA toolbox. This network was further modified to remove dead ends and include GPRs for modifiers, activators and inhibitors, resulting in the formation of the EGFR_SN network. The EGFR_SN network was constrained with the transcriptomic data of D492 and D492M to form EGFR_E and EGFR_M networks. Flux differences between EGFR_E and EGFR_M were used to further predict metabolic phenotype and target reactions critical for the reversal of mesenchymal to epithelial phenotype.