A Network Biology Approach Identifies Molecular Cross-Talk between Normal Prostate Epithelial and Prostate Carcinoma Cells
Fig 4
In vitro normal and tumour cell co-culture model.
The results of the in vitro cell co-culture experiment used to analyze normal and tumour epithelial cells crosstalk. (A) Table showing the number of overlapping genes between differentially regulated gene lists in normal and tumour cells as a result of co-culture. Rows in the table represent up and down regulated genes in normal cells whereas columns are up and down regulated genes in tumour cells. There is a significant overlap in gene lists changing in opposite directions (p<0.0001, red background). (B) PCA plot representing normal and tumour cells cultured on their own (RWPE1 and DU145) or in co-culture (RWPE1DU145 and DU145RWPE1). (C-D) Scatterplots comparing the number of genes in functional terms represented in the predicted targets of polarized genes and genes differentially expressed in the co-culture model. Panel C represent tumour cells whereas panel D represent normal cells.