APP Is a Context-Sensitive Regulator of the Hippocampal Presynaptic Active Zone
Fig 2
Quantitative immunodetection of hippocampal PAZ constituents derived from wild type and NexCre-cDKO mice.
Bar graphs are mean ± SEM, n = 3. *p<0.05, **p<0.01, n.s. difference not significant. The control (APLP2-KO, wt) was set to 100%. A representative Western blot is shown below each diagram. Equal amounts of protein (150 ng) were loaded per lane. APP (100±20%, 13±1%; 110 kDa), APLP1 (100±8%, 92±10%, 90 kDa), Munc18 (100±6%, 117±4%, 68 kDa), NCAM (100±11%, 94±28%, 140 and 180 kDa), SNAP25 (100±17%, 103±8%, 25 kDa), SV2A (100±2%, 127±7%, 86 kDa), synaptotagmin-1 (100±1%, 124±3%, Syt-1, 65 kDa), synaptophysin (100±7%, 97±14%, S-phys, 38 kDa), syntaxin1 (100±23%, 118±26%, 34 kDa), VAMP2 (100±1%, 106±4%, 18 kDa). Note that in NexCre-cDKO the deletion of APP is restricted to excitatory neurons.