APP Is a Context-Sensitive Regulator of the Hippocampal Presynaptic Active Zone
Fig 1
Overview of the experimental design.
A Workflow of subcellular fractionation and immunopurification of the native hippocampal PAZ. B Experimental outline of isobaric labeling of peptides with TMT6 and MS analysis by nano-high-pressure liquid chromatography (nHPLC-ESI). C Example of peptide signals (m/z) for the six reporter groups. D Differences in protein abundance of hippocampal PAZ constituents between APP-mutant and control. E Pie chart diagram of proteins attributed to the PAZ. F Scheme of a PPI network illustrating proteins (exemplarily designated as A-K) as nodes and edge betweeness. The thickness of the connections represents the importance of the respective edges for information flow within the network (edge betweenness). Change in abundance of more than ±10% is reflected by increasing sizes of nodes. The color code corresponds to the degree of up- (magenta) and downregulation (green). Nodes in yellow represent proteins with changes in abundance of less than ±10%. UF, upper fractions; LF, lower fractions; IP, immunopurification, MB, magnetic bead; PM, plasma membrane; SV, synaptic vesicle, SC, signaling cascade; CS, cytoskeleton; ME, metabolic enzymes; MI, mitochondria; O, others.