A New Fiji-Based Algorithm That Systematically Quantifies Nine Synaptic Parameters Provides Insights into Drosophila NMJ Morphometry
Fig 6
The influence of the abdominal body segments on NMJ features.
The plots display the mean (dot) and 95% confidence interval (blue error bars) of the NMJ values for each feature over the abdominal body segments A2 (n = 148), A3 (n = 356), A4 (n = 399) and A5 (n = 198). Four different categories are indicated by color. The blue category includes the features active zones (A), branches (B), branching points (C) and islands (D) and show a relative decrease from anterior to posterior with, except for the islands, A2 being significantly different. The second, orange, category includes the length (E) and longest branch length (F), showing an opposite pattern, in which A2 is significantly smaller than the other segments. The third category (green) includes the muscle area (G), that shows a significant increase in values from A2 to A3 and a significant decrease from A3 to A5. The last category (red) includes the features NMJ area (H) and perimeter (I), which shows a similarity between A2 and A5 versus A3-A4, only significant for NMJ area.