Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin
Fig 5
Kinetics of calnexin palmitoylation and stability.
A: The decay curves were predicted for the different palmitoylation species: fCAL represent folded calnexin, c1CAL and c2CAL the single palmitoylated species, c12CAL double palmitoylated calnexin. Solid line is the mean of the simulations of 382 models; shaded area is defined by the first and the third quartile of the simulations of 382 models (details of the in silico labelling experiment can be found in the Expanded View). B: Decay profiles were determined for WT calnexin either by 35S Cys/Meth labelling (35S calx WT) and by SNAP labeling (Calx WT SNAP). C: The model was simulated until it reached steady state and the values of the palmitoylation rates under steady state conditions were plotted. Error bars correspond to first and third quartile of the simulations of 382 models (details of the in silico labelling experiment can be found in the Expanded View). D: The model was simulated until it reached steady state and the values of the degradation rates under steady state conditions were plotted. Error bars were determined as in (C).