A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation
Table 3
Apparent Cas9 binding energies to canonical and non-canonical PAM sites (kcal/mol).
The energies are average values of all combinations in the first and fifth positions. (blue) The canonical PAM sites (NGGN) are bolded. N.B: no statistically significant binding. nt: nucleotide.