Systems-Wide Prediction of Enzyme Promiscuity Reveals a New Underground Alternative Route for Pyridoxal 5’-Phosphate Production in E. coli
Fig 3
Inactivating the thiG proposed secondary active site removes its ability to replace pdxB.
Four strains (ΔpdxB/empty [- control], ΔpdxB/pdxB [+ control], ΔpdxB/thiG, ΔpdxB/thiGmut) were grown for 96 hours in deep-well microplates, in which a checkerboard matrix of varied IPTG (inducer) and NH4Cl (nitrogen source) concentration in M9 glucose were assessed. Values shown are representative OD600 readings at 96 hours post-inoculation. Additional OD600 data are provided in S5 Table. Box plots of OD600 values at ~3% NH4Cl (across IPTG concentrations) are shown in lower panel, along with the results of Ranksum tests of OD600 values between relevant strain pairs.