The siRNA Non-seed Region and Its Target Sequences Are Auxiliary Determinants of Off-Target Effects
Fig 1
Graphical representation of siRNA molecule.
(A) siRNA duplex with presumed binding sites for RISC proteins, Ago and TRBP [6]. (B) Interaction pattern between siRNA guide strand and on-target transcript and (C) interaction between siRNA guide strand and its seed-dependent off-target transcript. The mechanistic overview shows the guide strand of siRNA (A) as the strand which enters RISC, and acts in downstream silencing events (B and C). Nucleotide position numbers mirror those used in the text and consequent figures.