MIiSR: Molecular Interactions in Super-Resolution Imaging Enables the Analysis of Protein Interactions, Dynamics and Formation of Multi-protein Structures
Fig 1
MIiSR graphical user interfaces and work flow.
(A) Image of the MIiSRconvert GUI, which is used to convert microscope position files from a manufacturer’s format into a MIiSR-compatible format. A complete description of use of the GUI can be found in its help file. The example GUI has had multiple Leica SR GSD position files loaded into the queue for processing. (B) Image of the MIiSR GUI, which is used to perform the analyses described in this paper on super-resolution position files generated by MIiSRconvert. Four ROIs from the current image have been added to the queue. A complete description of the use of the GUI can be found in its help file, and all assays are described in this paper. (C) Workflow of MIiSR analysis. Solid arrows indicate mandatory steps, dotted arrows and italicized text indicate optional steps.