A Jump-from-Cavity Pyrophosphate Ion Release Assisted by a Key Lysine Residue in T7 RNA Polymerase Transcription Elongation
Fig 1
The modeled PPi-bound T7 RNA elongation complex.
(A) The overall structure of the complex (protein in white; DNA non-template strand in purple; DNA template strand in green; RNA strand in yellow). (B) The zoomed in view of the active site, with essential amino acids shown in the upper panel (blue licorice: positively charged; red licorice: negatively charged). PPi is shown in red spheres. The Mg2+ in the active site is in green sphere. The O-helix is shown in cyan and the PPi release channel is highlighted in wheat color. The lower panel provides a surface representation.