Inferring the Forces Controlling Metaphase Kinetochore Oscillations by Reverse Engineering System Dynamics
Fig 8
Force profiles during directional switches.
Time-dependent estimation of (A) spring force and (B) PEF. Switch events from trajectories where the lead (LIDS, n = 1614; magenta) or trail (TIDS, n = 449; black) kinetochore switched first were aligned at their median switching time (the time origin is marked by vertical blue line). Solid lines indicate mean, dashed lines ±s.e.m.. Forces are given as velocities by rescaling by the viscosity coefficient. (C) Opposing force (spring + PEF) on lead sister during a LIDS (magenta) or TIDS (black) is plotted relative to the depolymerisation force F−. Solid lines indicate mean, dashed lines 5% and 95% percentiles of the population. (D) Spring force heat map across switching events partitioned by difference between probability of LIDS (pLIDS) or TIDS (pTIDS). Events above zero on right y-axis are classified as LIDS; those below as TIDS.