Inferring the Forces Controlling Metaphase Kinetochore Oscillations by Reverse Engineering System Dynamics
Fig 4
Population parameter analysis.
Posterior means for (A) K-fibre (de)polymerisation velocities v+ (blue) and v− (red); (B) spring constant κ; (C) anti-poleward force gradient α; (D) noise parameter τ = s−2; (E) natural length L (blue); (F) the probabilities of not switching state per time-point when sisters are coherent (blue) and incoherent (red). The informed Gaussian prior on L is shown in red in (E). Dashed line and shaded areas in (A-F) indicate mean and ±s.d., respectively. (G-L) Trajectories binned by radial distance from the centre of metaphase plate (with approximately equal numbers per bin; alternating grey and white boxes) for parameters as in (A-F). Circles indicate mean of bin and lines show ±s.d.. All panels include all converged and filtered trajectories (n = 843).