Regulation of Early Steps of GPVI Signal Transduction by Phosphatases: A Systems Biology Approach
Fig 6
Profiles obtained from fitting Model C to experimental observations.
Model profiles obtained from fitting Model C to experimental observation describing Syk phosphorylation on Y525 and Y323 are shown in panels a and b. Model C with H3 implemented (solid lines) faithfully represents the experimental data. Model C with H1 or H2 implemented (broken and dotted lines respectively) fail to describe the full dynamics displayed in the data. Model C fitted to a restricted set of experimental observation, describing Syk phosphorylation on Y525 closely replicates observations describing Y525 (panel c) but model predictions for Y323 phosphorylation (panel d) are nearly three fold higher than that seen in experimental data. In all panels experimental data of Syk phosphorylation (molecules per platelet) is the mean of three samples +/- S.E.M., phosphorylated (circles). Model simulations (H1, broken lines; H2, dotted lines; H3, solid lines) shown in panels a and b utilise parameter values from Table J in S1 Text (parameter set 1) and simulations shown in panels c and d utilise values from Table I in S1 Text (parameter set 1).