Fluctuation-Driven Neural Dynamics Reproduce Drosophila Locomotor Patterns
Fig 1
A high-resolution, high-throughput assay for measuring Drosophila locomotor patterns.
(A) Schematic of planar behavioral arenas. Laminar flow of air or odor (10% acetic acid) is presented to either or both halves of the arena. Colored arrows indicate flow inlets and outlets (green/blue and red, respectively). (B) Camera-view of five experimental arenas and behavior tracking. Each fly is represented as a colored triangle. A colored line represents a fly’s location during the previous 10 s. (C) Schematic time-course of the behavioral experiment. We studied basal and odor-evoked locomotion as well as odor aversion for each fly. While not shown, during minutes 3–4.5, air flowed throughout the arena. (D) Speed time-series (black) were transformed into binary ‘Walking’ or ‘Stationary’ time-series (red). (E) Representative locomotor traces for five Canton-S strain flies during the odor impulse experiment. Flies were exposed to 60 s of air flow, 30 s of odor throughout the arena, and then 90 s of air flow. Behavior for each fly is shown in red. High values indicate walking while low values indicate stationary periods. (F) Locomotor traces averaged across 225 Canton-S flies during the odor impulse experiment. Prior to odor stimulation (grey bar) there is basal locomotion (green) followed by decay in locomotor frequency (cyan) to a reduced level of basal locomotion (magenta).