SINCERA: A Pipeline for Single-Cell RNA-Seq Profiling Analysis
Fig 3
Validation of Cell Type Assignments using Known Biomarkers.
(A) Expression patterns of representative known cell type markers were used to validate the correct assignment of major lung cell types at E16.5. Expression levels were normalized by per-sample z-score transformation. (B) ROC curves of the rank-aggregation-based validation showed a high consistency (AUC>0.8) between the cell type assignments and the expression patterns of known cell type specific markers (S2 Table).