An Ovol2-Zeb1 Mutual Inhibitory Circuit Governs Bidirectional and Multi-step Transition between Epithelial and Mesenchymal States
Fig 5
Stepwise induction of EMT in MCF10A cells by different doses of TGF-β.
A) Cells were treated with various concentrations of TGF-β for 10 days and analyzed for Ecad/Vim expression by flow cytometry. Each panel shows a superimposed image of two treatment conditions. Note that the 25 μM TGF-β treatment gave rise to a heterogeneous population containing I2 cells and M cells (orange). B) The corresponding steady states of the cellular phenotypes (indicated as colored dots) observed under various TGF-β concentrations in A are mapped to the bifurcation diagram shown in Fig 2B. C) An illustrative summary of phenotypic transitions in the four-state system. Solid arrow represents transition with experimental evidence from this study. Dotted arrow represents hypothetical transition without experimental evidence.