High-Specificity Targeted Functional Profiling in Microbial Communities with ShortBRED
Fig 2
Accuracy of ShortBRED and centroid-based profiling within synthetic metagenomes.
(A, B) ROC curves report the sensitivity and specificity (in terms of TPR and FPR) of the two methods for correctly identifying the presence and absence of protein families of interest in six synthetic metagenomes, spiked with 5%, 10%, and 25% of their material from the ARDB (panel A) and VFDB (panel B). (C, D) Scatterplots of protein family “predicted from mapping”, the abundance values calculated by ShortBRED and the centroids, versus “expected from gold standard”, the abundance values of the protein families in the 10% synthetic metagenome.