FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants
Fig 3
Biochemical properties of DhaA wild-type and the final mutant DhaA115.
A) Melting temperatures of DhaA wild-type (blue) and DhaA115 (red) in the presence of indicated solvents. B) Half-life of DhaA wild-type (blue) and DhaA115 (red) determined at 60°C and pH 8.6 with the substrate 1-iodohexane. C) Temperature profiles of DhaA wild-type (blue) and DhaA115 (red) determined at pH 8.6 with the substrate 1-iodohexane.