FireProt: Energy- and Evolution-Based Computational Design of Thermostable Multiple-Point Mutants
Fig 2
Location of stabilizing mutations in designed enzymes.
A) Locations of substitutions in energy-based, evolution-based and combined mutants of DhaA enzyme. Substitutions in the multiple-point mutant designed by the energy-based approach (DhaA112) are represented as orange spheres, while substitutions in multiple-point mutants designed by the evolution-based approach are represented as red (DhaA100), blue (DhaA101), green (DhaA102) and magenta (DhaA103) spheres. Mutations in the combined mutant (DhaA115) are colored in orange and blue in correspondence with their original mutants (DhaA112 and DhaA101). B) Locations of substitutions in energy-based, and evolution-based mutants of LinA enzyme. Substitutions in the multiple-point mutant designed by the energy-based approach (LinA01) are represented as orange spheres, while substitutions in multiple-point mutant designed by the evolution-based approach (LinA02) are represented as blue spheres.