Efficient Optimization of Stimuli for Model-Based Design of Experiments to Resolve Dynamical Uncertainty
Fig 2
An illustration of the optimal experiment design for the Hes1 example.
(a) The input sequence selected by the design for the duration of the experiment. (b) Model simulation of the output dynamics of the measurable species m and P1+P2 under an optimal input simulated with ΩRA. The red dots specify the selected optimal measurement points which are the mean values generated using nominal plant with additive 10% Gaussian noise. Error bars are also given by the standard deviation of the simulated data (three data points are generated for each time point). (c) The initial (grey) and final (blue) uncertainty in the target states dynamics. The designed experiment reduces the uncertainty region by 84%,81%, and 86% for mRNA, P1, and P2, respectively. The red line shows the simulated dynamics of the nominal plant.