Comprehensive Meta-analysis of Ontology Annotated 16S rRNA Profiles Identifies Beta Diversity Clusters of Environmental Bacterial Communities
Fig 1
Overview of Microbial Community meta-analysis.
The diagram lists all major components of our framework and their relation to each other. The results of each step are shown next to each component. Tools/scripts are shown in type writer font. a) Data acquisition and database creation: we collect data samples from four different sources and unify there representations such that they can be integrated in a single relational database. b) The given web page provides a user-friendly way to generate highly customized BIOM tables to facilitate user-specific meta-analyses. c) Adaptive rarefaction, taking input either from the database or from a BIOM table, produces all-against-all beta diversity distance matrices for the provided samples. d) Conventional hierarchical clustering. e) Posthoc enrichment test for EnvO annotations. The final output are a spreadsheet, documenting enriched clusters (precision, recall, F-measure, cluster coefficients, EnvO-terms, etc.), and interactive, annotated clustering visualizations.