Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data

doi:10.1371/journal.pcbi.1004412

Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data

Fig 10

Waste & under-oxygenation mediating quiescence.

If a cell was exposed to waste (cellular debris from cellular lyses of dead cells) or deprived from oxygen for a certain number of cell cycles, , it will become quiescent after its next division. The population kinetics of all conditions I-IV during all growth phases including the saturation as well as the spatial temporal profiles for conditions III ([G] = 25mM, [O] = 0.28mM), and II ([G] = 5mM, [O] = 0.28mM) were correctly captured. The graphs show a comparison between experimental observation and model simulation. The experimental growth curves are either shown as boxplots (box: mean, lower & upper quartiles, horizontal dashes: minimum & maximum), or as composition of mean (line) and standard deviation (error bar) for the radial profiles. The simulation curves are shown as black lines.

doi: https://doi.org/10.1371/journal.pcbi.1004412.g010