Inferring Growth Control Mechanisms in Growing Multi-cellular Spheroids of NSCLC Cells from Spatial-Temporal Image Data
Fig 7
Growth kinetics (upper panel) and spatial profiles of TUNEL, Ki67, Collagen IV for [G] = 5mM, [O] = 0.28mM (condition II, upper right picture and middle panel) and [G] = 25mM, [O] = 0.28mM (condition III, upper left picture and low panel.).
The dotted curves show simulations where cell cycle progression requires the product of local glucose and oxygen concentration to exceed a critical threshold, while below this threshold cells become quiescent and die. If the product is replaced by the local concentration of ATP, the agreement improves significantly (solid lines). For dashed curves the diffusion coefficient in medium was assumed to be Dmed = 30Dtum. The experimental growth curves are shown as boxplots (box: mean, lower & upper quartiles, horizontal dashes: minimum & maximum), and the radial profiles as composition of mean (bold line) and standard deviation (thin line).