Consistency of VDJ Rearrangement and Substitution Parameters Enables Accurate B Cell Receptor Sequence Annotation
Fig 3
Mutation frequency versus position.
Typical observed mutation frequencies for two V, two D, and two J alleles on the three humans (A, B, and C) in the Adaptive data set. The x axis is the zero-indexed position along the IMGT germline allele. Mutation frequencies are seen to be highly position-dependent. While the structure of these mutation distributions appears similar between humans, the overall level of mutation varies. The first base of the conserved cysteine and tryptophan codons (i.e. the CDR3 boundaries) are indicated with black vertical dashed lines. In the complete set of plots (which are publicly available as described in the text), mutation frequencies are highly variable across sites with a pattern that is similar between humans.